human PUM1 shRNA silencing AAV

Name 
AAV-h-PUM1-shRNA
Cat No 
shAAV-220392
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PUM1.

shAAV-220392
AAV-h-PUM1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PUM1 (pumilio RNA binding family member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HSPUM; PUMH; PUMH1; pumilio homolog 1; pumilio-1; PUML1; SCA47
Description (eCommerce)
Pumilio 1 is an evolutionarily conserved RNA binding protein that functions as a translational regulator of genes involved in embryogenesis and germline cell development. Pumilio 1, which is expressed in many tissue types, is homologous to the germline specific pumilio 2.
Description (Vector)
PUM1 encodes a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. The encoded protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of the encoded protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID
9698
Gene Name (eCommerce)
pumilio RNA binding family member 1
Gene Name (Vector)
pumilio RNA binding family member 1
Gene Symbol
PUM1
HGNC ID
HGNC:14957
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3561
ORF Size (bp)
3561 bp
Protein Name (eCommerce)
Pumilio homolog 1
RefSeq ID
NM_014676
RefSeq Synonyms
NM_014676, NM_001020658, BC013398,
Research Areas
Golgi Apparatus,RNA Binding,Translational Control
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
Pumilio 1 is an evolutionarily conserved RNA binding protein that functions as a translational regulator of genes involved in embryogenesis and germline cell development. Pumilio 1, which is expressed in many tissue types, is homologous to the germline specific pumilio 2.
UniGene ID
Hs.281707
UniProt ID (eCommerce)
Q14671

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.