human PRKCI shRNA silencing AAV

Name
AAV-h-PRKCI-shRNA

Cat No
shAAV-219866

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PRKCI.

shAAV-219866

AAV-h-PRKCI-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRKCI (protein kinase C iota). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

DXS1179E; nPKC-iota; PKCI

Description (Vector)

PRKCI encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Gene ID

5584

Gene Name (Vector)

protein kinase C iota

Gene Symbol

PRKCI

HGNC ID

HGNC:9404

ORF Size (aa)

1764

ORF Size (bp)

1764 bp

RefSeq ID

NM_002740

RefSeq Synonyms

NM_002740, BC022016,

Species

human

UniGene ID

Hs.478199

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.