human PRKCE shRNA silencing AAV

Name
AAV-h-PRKCE-shRNA

Cat No
shAAV-219862

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PRKCE.

shAAV-219862

AAV-h-PRKCE-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRKCE (protein kinase C epsilon). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

nPKC-epsilon; PKCE

Description (Vector)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Gene ID

5581

Gene Name (Vector)

protein kinase C epsilon

Gene Symbol

PRKCE

HGNC ID

HGNC:9401

ORF Size (aa)

2214

ORF Size (bp)

2214 bp

RefSeq ID

NM_005400

RefSeq Synonyms

NM_005400, BC109034, BC109033,

Species

human

UniGene ID

Hs.580351

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.