human PRKAB2 shRNA silencing AAV

Name
AAV-h-PRKAB2-shRNA

Cat No
shAAV-219839

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PRKAB2.

shAAV-219839

AAV-h-PRKAB2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRKAB2 (protein kinase AMP-activated non-catalytic subunit beta 2). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

PRKAB2 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

Gene ID

5565

Gene Name (Vector)

protein kinase AMP-activated non-catalytic subunit beta 2

Gene Symbol

PRKAB2

HGNC ID

HGNC:9379

ORF Size (aa)

819

RefSeq ID

NM_005399

RefSeq Synonyms

NM_005399, BC053610,

Species

human

UniGene ID

Hs.50732

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.