human PRKAB1 shRNA silencing AAV

Name
AAV-h-PRKAB1-shRNA

Cat No
shAAV-219835

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PRKAB1.

shAAV-219835

AAV-h-PRKAB1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRKAB1 (protein kinase AMP-activated non-catalytic subunit beta 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AMPK; HAMPKb

Description (Vector)

PRKAB1 is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Gene ID

5564

Gene Name (Vector)

protein kinase AMP-activated non-catalytic subunit beta 1

Gene Symbol

PRKAB1

HGNC ID

HGNC:9378

ORF Size (aa)

813

RefSeq ID

NM_006253

RefSeq Synonyms

NM_006253, BC017671, BC001823, BC001056, BC001007,

Species

human

UniGene ID

Hs.715515

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.