human PRKAA2 shRNA silencing AAV

Name 
AAV-h-PRKAA2-shRNA
Cat No 
shAAV-219832
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PRKAA2.

shAAV-219832
AAV-h-PRKAA2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRKAA2 (protein kinase AMP-activated catalytic subunit alpha 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; acetyl-CoA carboxylase kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMPK; AMPK alpha 2; AMPK subunit alpha-2; AMPK2; AMPKa2; AMPK-alpha-2 chain; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; PRKAA; protein kinase, AMP-activated, alpha 2 catalytic subunit
Description (eCommerce)
AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 2 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted.
Description (Vector)
PRKAA2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Gene ID
5563
Gene Name (eCommerce)
protein kinase AMP-activated catalytic subunit alpha 2
Gene Name (Vector)
protein kinase AMP-activated catalytic subunit alpha 2
Gene Symbol
PRKAA2
HGNC ID
HGNC:9377
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1659
ORF Size (bp)
1659 bp
Protein Name (eCommerce)
5'-AMP-activated protein kinase catalytic subunit alpha-2
RefSeq ID
NM_006252
RefSeq Synonyms
NM_006252, BC069823, BC069740, BC069680,
Research Areas
Apoptosis,Autophagy,BDNF Signaling,Cancer,Cardiology,Cardiomyopathy,Cardiovascular,Cell Cycle,EGFR Signaling,EPO Signaling,IGF-1 Signaling,Insulin Signaling,Interleukin Signaling,JAK-STAT Signaling,Kinase/Phosphatase,Leptin Signaling,LKB1 Signaling,Metabolism/Metabolic Process,mTOR Signaling,Neurobiology,PI3K/Akt Signaling,Signal Transduction,Transcription Factor/Regulator,VEGF Signaling,Wnt Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,genetics,immunology,metabolism,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 2 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted.
UniGene ID
Hs.437039
UniProt ID (eCommerce)
P54646

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.