human PPT1 shRNA silencing AAV

Name
AAV-h-PPT1-shRNA

Cat No
shAAV-219694

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PPT1.

shAAV-219694

AAV-h-PPT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPT1 (palmitoyl-protein thioesterase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CLN1; INCL; PPT

Description (Vector)

PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.

Gene ID

5538

Gene Name (Vector)

palmitoyl-protein thioesterase 1

Gene Symbol

PPT1

HGNC ID

HGNC:9325

ORF Size (aa)

921

RefSeq ID

NM_000310

RefSeq Synonyms

NM_001142604, NM_000310, BC008426,

Species

human

UniGene ID

Hs.3873

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.