human PPP1R12B shRNA silencing AAV

Name 
AAV-h-PPP1R12B-shRNA
Cat No 
shAAV-219609
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PPP1R12B.

shAAV-219609
AAV-h-PPP1R12B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPP1R12B (protein phosphatase 1 regulatory subunit 12B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
MYPT2; PP1bp55
Description (Vector)
Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome.
Gene ID
4660
Gene Name (Vector)
protein phosphatase 1 regulatory subunit 12B
Gene Symbol
PPP1R12B
HGNC ID
HGNC:7619
ORF Size (aa)
1548
ORF Size (bp)
1548 bp
RefSeq ID
NM_002481
RefSeq Synonyms
NM_032104, NM_032103, NM_002481, NM_001331029, NM_001197131, NM_001167858, NM_001167857, BC144699, BC110907, BC071166,
Species
human
UniGene ID
Hs.444403

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.