human PPM1D shRNA silencing AAV

Name
AAV-h-PPM1D-shRNA

Cat No
shAAV-219575

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PPM1D.

shAAV-219575

AAV-h-PPM1D-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPM1D (protein phosphatase, Mg2+/Mn2+ dependent 1D). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PP2C-DELTA; WIP1

Description (Vector)

PPM1D is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Gene ID

8493

Gene Name (Vector)

protein phosphatase, Mg2+/Mn2+ dependent 1D

Gene Symbol

PPM1D

HGNC ID

HGNC:9277

ORF Size (aa)

1818

ORF Size (bp)

1818 bp

RefSeq ID

NM_003620

RefSeq Synonyms

NM_003620, BC060877, BC042418, BC033893, BC016480,

Species

human

UniGene ID

Hs.591184

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.