human PPM1A shRNA silencing AAV

Name
AAV-h-PPM1A-shRNA

Cat No
shAAV-219571

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PPM1A.

shAAV-219571

AAV-h-PPM1A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPM1A (protein phosphatase, Mg2+/Mn2+ dependent 1A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PP2C-ALPHA; PP2CA; PP2Calpha

Description (Vector)

PPM1A is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Gene ID

5494

Gene Name (Vector)

protein phosphatase, Mg2+/Mn2+ dependent 1A

Gene Symbol

PPM1A

HGNC ID

HGNC:9275

ORF Size (aa)

1149

ORF Size (bp)

1149 bp

RefSeq ID

NM_021003

RefSeq Synonyms

NM_177952, NM_177951, NM_021003, BC063243, BC026691,

Species

human

UniGene ID

Hs.130036

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.