human PPIF shRNA silencing AAV

Name 
AAV-h-PPIF-shRNA
Cat No 
shAAV-219559
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PPIF.

shAAV-219559
AAV-h-PPIF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPIF (peptidylprolyl isomerase F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Cyp-D; CyP-M; CYP3; CypD
Description (Vector)
PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Gene ID
10105
Gene Name (Vector)
peptidylprolyl isomerase F
Gene Symbol
PPIF
HGNC ID
HGNC:9259
ORF Size (aa)
468
RefSeq ID
NM_005729
RefSeq Synonyms
NM_005729, BC110299, BC005020,
Species
human
UniGene ID
Hs.381072

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.