human POMT1 shRNA silencing AAV

Name
AAV-h-POMT1-shRNA

Cat No
shAAV-219437

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human POMT1.

shAAV-219437

AAV-h-POMT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human POMT1 (protein O-mannosyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

LGMD2K; MDDGA1; MDDGB1; MDDGC1; RT

Description (Vector)

POMT1 is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.

Gene ID

10585

Gene Name (Vector)

protein O-mannosyltransferase 1

Gene Symbol

POMT1

HGNC ID

HGNC:9202

ORF Size (aa)

2178

ORF Size (bp)

2178 bp

RefSeq ID

NM_007171

RefSeq Synonyms

NM_007171, NM_001136114, NM_001136113, NM_001077366, NM_001077365, BC065268, BC022877,

Species

human

UniGene ID

Hs.522449

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.