human PIWIL1 shRNA silencing AAV

Name
AAV-h-PIWIL1-shRNA

Cat No
shAAV-219010

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PIWIL1.

shAAV-219010

AAV-h-PIWIL1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PIWIL1 (piwi like RNA-mediated gene silencing 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CT80.1; HIWI; MIWI; PIWI

Description (Vector)

PIWIL1 encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

9271

Gene Name (Vector)

piwi like RNA-mediated gene silencing 1

Gene Symbol

PIWIL1

HGNC ID

HGNC:9007

ORF Size (aa)

2586

ORF Size (bp)

2586 bp

RefSeq ID

NM_004764

RefSeq Synonyms

NM_004764, NM_001190971, BC028581,

Species

human

UniGene ID

Hs.405659

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.