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human PHGDH shRNA silencing AAV

Name 
AAV-h-PHGDH-shRNA
Cat No 
shAAV-218825
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PHGDH.

shAAV-218825
AAV-h-PHGDH-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PHGDH (phosphoglycerate dehydrogenase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2-oxoglutarate reductase; 3PGDH; 3-PGDH; 3-phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; HEL-S-113; malate dehydrogenase; NLS; NLS1; PDG; PGAD; PGD; PGDH; PHGDHD; SERA
Description (eCommerce)
D-3-phosphoglycerate dehydrogenase (PHGDH) is an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptom [taken from NCBI Entrez Gene (Gene ID: 26227)].
Description (Vector)
PHGDH encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
Gene ID
26227
Gene Name (eCommerce)
phosphoglycerate dehydrogenase
Gene Name (Vector)
phosphoglycerate dehydrogenase
Gene Symbol
PHGDH
HGNC ID
HGNC:8923
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1602
ORF Size (bp)
1602 bp
Protein Name (eCommerce)
D-3-phosphoglycerate dehydrogenase
RefSeq ID
NM_006623
RefSeq Synonyms
NM_006623, BC011262, BC001349, BC000303,
Research Areas
Brain Development,Metabolism/Metabolic Process,Neurobiology,Neurodevelopment
Research Areas (Faceted)
cell_biology,developmental_biology,metabolism,neurobiology
Species
human
Target Sentence
D-3-phosphoglycerate dehydrogenase (PHGDH) is an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptom [taken from NCBI Entrez Gene (Gene ID: 26227)].
UniGene ID
Hs.487296
UniProt ID (eCommerce)
O43175

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.