human PHB shRNA silencing AAV

Name 
AAV-h-PHB-shRNA
Cat No 
shAAV-218784
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PHB.

shAAV-218784
AAV-h-PHB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PHB (prohibitin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
epididymis luminal protein 215; epididymis secretory sperm binding protein Li 54e; HEL-215; HEL-S-54e; PHB1; prohibitin
Description (eCommerce)
Prohibitin (PHB) has been identified as a tumor suppressor protein that can repress E2F-mediated transcription and arrest cell cycle progression. It has recently been shown to associate with members of the minichromosome maintenance complex such as MCM2, MCM5, and MCM7 and therefore is thought to impart its growth inhibitory effects via association with the replication machinery. Prohibitin is also implicated in regulating mitochondrial respiration and playing a role in aging.
Description (Vector)
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Gene ID
5245
Gene Name (eCommerce)
prohibitin
Gene Name (Vector)
prohibitin
Gene Symbol
PHB
HGNC ID
HGNC:8912
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
819
Protein Name (eCommerce)
Prohibitin
RefSeq ID
NM_002634
RefSeq Synonyms
NM_002634, NM_001281715, NM_001281497, NM_001281496, BC095460, BC013401,
Research Areas
Apoptosis,Breast Cancer,Cancer,DNA Replication,Histone Modification,Interleukin Signaling,Mitochondrion,RNA Binding,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,genetics,immunology,signal_transduction,transcription_translation
Species
human
Target Sentence
Prohibitin (PHB) has been identified as a tumor suppressor protein that can repress E2F-mediated transcription and arrest cell cycle progression. It has recently been shown to associate with members of the minichromosome maintenance complex such as MCM2, MCM5, and MCM7 and therefore is thought to impart its growth inhibitory effects via association with the replication machinery. Prohibitin is also implicated in regulating mitochondrial respiration and playing a role in aging.
UniGene ID
Hs.514303
UniProt ID (eCommerce)
P35232

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.