human PDHA1 shRNA silencing AAV

Name
AAV-h-PDHA1-shRNA

Cat No
shAAV-218511

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PDHA1.

shAAV-218511

AAV-h-PDHA1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PDHA1 (pyruvate dehydrogenase (lipoamide) alpha 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PDHA; PDHAD; PDHCE1A; PHE1A

Description (Vector)

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

5160

Gene Name (Vector)

pyruvate dehydrogenase (lipoamide) alpha 1

Gene Symbol

PDHA1

HGNC ID

HGNC:8806

ORF Size (aa)

1173

RefSeq ID

NM_000284

RefSeq Synonyms

NM_001173456, NM_001173455, NM_001173454, NM_000284, BC002406,

Species

human

UniGene ID

Hs.530331

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.