human PDE6B shRNA silencing AAV

Name
AAV-h-PDE6B-shRNA

Cat No
shAAV-218483

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human PDE6B.

shAAV-218483

AAV-h-PDE6B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PDE6B (phosphodiesterase 6B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CSNB3; CSNBAD2; PDEB; rd1; RP40

Description (Vector)

Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

5158

Gene Name (Vector)

phosphodiesterase 6B

Gene Symbol

PDE6B

HGNC ID

HGNC:8786

ORF Size (aa)

2562

ORF Size (bp)

2562 bp

RefSeq ID

NM_000283

RefSeq Synonyms

NM_001145292, NM_001145291, NM_000283, BC000249,

Species

human

UniGene ID

Hs.654544

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.