human PDE6B shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human PDE6B.
shAAV-218483
AAV-h-PDE6B-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human PDE6B (phosphodiesterase 6B). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CSNB3; CSNBAD2; PDEB; rd1; RP40
Description (Vector)
Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
5158
Gene Name (Vector)
phosphodiesterase 6B
Gene Symbol
PDE6B
HGNC ID
HGNC:8786
ORF Size (aa)
2562
ORF Size (bp)
2562 bp
RefSeq ID
NM_000283
RefSeq Synonyms
NM_001145292, NM_001145291, NM_000283, BC000249,
Species
human
UniGene ID
Hs.654544
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.