human PCBP2 shRNA silencing AAV

Name
AAV-h-PCBP2-shRNA

Cat No
shAAV-218307

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PCBP2.

shAAV-218307

AAV-h-PCBP2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PCBP2 (poly(rC) binding protein 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

hnRNP-E2; HNRNPE2; HNRPE2

Description (Vector)

PCBP2 appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

5094

Gene Name (Vector)

poly(rC) binding protein 2

Gene Symbol

PCBP2

HGNC ID

HGNC:8648

ORF Size (aa)

1008

ORF Size (bp)

1008 bp

RefSeq ID

NM_005016

RefSeq Synonyms

NM_031989, NM_005016, NM_001128914, NM_001128913, NM_001128912, NM_001128911, NM_001098620, BC107688, BC071942, BC001155,

Species

human

UniGene ID

Hs.546271

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.