human ORC1L shRNA silencing AAV

Name
AAV-h-ORC1L-shRNA

Cat No
shAAV-217924

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ORC1.

shAAV-217924

AAV-h-ORC1L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ORC1 (origin recognition complex subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HSORC1; ORC1L; origin recognition complex subunit 1; origin recognition complex, subunit 1 homolog; PARC1; replication control protein 1

Description (eCommerce)

ORC1 (origin recognition complex, subunit 1) is one of six subunits that make up the origin of recognition complex (ORC). The ORC serves as a scaffold for proteins involved in the initiation of DNA replication. Very recently, ORC1 has been found to perform a function independent of its role in DNA replication. In a screen using siRNAs for human ORC proteins to assess their roles in centrosome biology, it was found that ORC1 plays a key role in controlling centriole and centrosome copy number in human cells. ORC1 is also known as replication control protein 1, ORC1L, HSORC1, and PARC1.

Description (Vector)

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene ID

4998

Gene Name (eCommerce)

origin recognition complex subunit 1

Gene Name (Vector)

origin recognition complex subunit 1

Gene Symbol

ORC1

HGNC ID

HGNC:8487

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2586

ORF Size (bp)

2586 bp

Protein Name (eCommerce)

Origin recognition complex subunit 1

RefSeq ID

NM_004153

RefSeq Synonyms

NM_004153, NM_001190819, NM_001190818, BC011539,

Research Areas

Cell Cycle,Cell Division,DNA Replication,Replication

Research Areas (Faceted)

cell_biology,cell_cycle,genetics,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.17908

UniProt ID (eCommerce)

Q13415

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.