human NSDHL shRNA silencing AAV

Name 
AAV-h-NSDHL-shRNA
Cat No 
shAAV-217110
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NSDHL.

shAAV-217110
AAV-h-NSDHL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NSDHL (NAD(P) dependent steroid dehydrogenase-like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
H105E3; SDR31E1; XAP104
Description (Vector)
NSDHL is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Gene ID
50814
Gene Name (Vector)
NAD(P) dependent steroid dehydrogenase-like
Gene Symbol
NSDHL
HGNC ID
HGNC:13398
ORF Size (aa)
1122
ORF Size (bp)
1122 bp
RefSeq ID
NM_015922
RefSeq Synonyms
NM_015922, NM_001129765, BC007816, BC000245,
Species
human
UniGene ID
Hs.57698

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.