human NP shRNA silencing AAV

Name 
AAV-h-NP-shRNA
Cat No 
shAAV-216928
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PNP.

shAAV-216928
AAV-h-NP-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PNP (purine nucleoside phosphorylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
epididymis secretory sperm binding protein Li 156an; HEL-S-156an; inosine phosphorylase; inosine-guanosine phosphorylase; NP; PNP; PRO1837; PUNP; purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase
Description (eCommerce)
Purine nucleoside phosphorylase (PNP) is an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The PNP enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described [taken from NCBI Entrez Gene (Gene ID: 4860)].
Description (Vector)
PNP encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
Gene ID
4860
Gene Name (eCommerce)
purine nucleoside phosphorylase
Gene Name (Vector)
purine nucleoside phosphorylase
Gene Symbol
PNP
HGNC ID
HGNC:7892
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
870
Protein Name (eCommerce)
Purine nucleoside phosphorylase
RefSeq ID
NM_000270
RefSeq Synonyms
NM_000270, BC106074, BC104207, BC104206,
Research Areas
Cytoskeleton,Immunology,Metabolism/Metabolic Process
Research Areas (Faceted)
cell_biology,immunology,metabolism
Species
human
Target Sentence
Purine nucleoside phosphorylase (PNP) is an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The PNP enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described [taken from NCBI Entrez Gene (Gene ID: 4860)].
UniGene ID
Hs.75514
UniProt ID (eCommerce)
P00491

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.