human NOC2L shRNA silencing AAV

Name 
AAV-h-NOC2L-shRNA
Cat No 
shAAV-216850
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NOC2L.

shAAV-216850
AAV-h-NOC2L-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NOC2L (NOC2 like nucleolar associated transcriptional repressor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
NET15; NET7; NIR; NOC2L nucleolar associated transcriptional repressor; NOC2-like protein; novel INHAT (inhibitor of histone acetyltransferase) repressor; novel INHAT repressor; nucleolar complex associated 2 homolog; nucleolar complex protein 2 homolog; PPP1R112; protein NOC2 homolog; protein phosphatase 1, regulatory subunit 12
Description (eCommerce)
Novel INHAT repressor (NIR) acts as an inhibitor of histone acetyltransferase activity; it prevents acetylation of all core histones by the EP300/p300 histone acetyltransferase at p53/TP53-regulated target promoters in a histone deacetylases (HDAC)-independent manner. NIR acts as a transcription corepressor of p53/TP53- and TP63-mediated transactivation of the p21/CDKN1A promoter. It is involved in the regulation of p53/TP53-dependent apoptosis and it associates together with TP63 isoform TA*-gamma to the p21/CDKN1A promoter [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3T9].
Description (Vector)
Histone modification by histone acetyltransferases (HAT) and histone deacetylases (HDAC) can control major aspects of transcriptional regulation. NOC2L represents a novel HDAC-independent inhibitor of histone acetyltransferase (INHAT) (Hublitz et al., 2005 (PubMed 16322561)).(supplied by OMIM, Mar 2008)
Gene ID
26155
Gene Name (eCommerce)
NOC2 like nucleolar associated transcriptional repressor
Gene Name (Vector)
NOC2 like nucleolar associated transcriptional repressor
Gene Symbol
NOC2L
HGNC ID
HGNC:24517
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2250
ORF Size (bp)
2250 bp
Protein Name (eCommerce)
Nucleolar complex protein 2 homolog
RefSeq ID
NM_015658
RefSeq Synonyms
NM_015658, BC003555,
Research Areas
Apoptosis,Chromatin Remodeling,Histone Modification,Immunology,Kinase/Phosphatase,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,genetics,immunology,signal_transduction,transcription_translation
Species
human
Target Sentence
Novel INHAT repressor (NIR) acts as an inhibitor of histone acetyltransferase activity; it prevents acetylation of all core histones by the EP300/p300 histone acetyltransferase at p53/TP53-regulated target promoters in a histone deacetylases (HDAC)-independent manner. NIR acts as a transcription corepressor of p53/TP53- and TP63-mediated transactivation of the p21/CDKN1A promoter. It is involved in the regulation of p53/TP53-dependent apoptosis and it associates together with TP63 isoform TA*-gamma to the p21/CDKN1A promoter [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3T9].
UniGene ID
Hs.405987
UniProt ID (eCommerce)
Q9Y3T9

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.