human NMT1 shRNA silencing AAV

Name 
AAV-h-NMT1-shRNA
Cat No 
shAAV-216836
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NMT1.

shAAV-216836
AAV-h-NMT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NMT1 (N-myristoyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
alternative, short form NMT-S; glycylpeptide N-tetradecanoyltransferase 1; long form, NMT-L; myristoyl-CoA:protein N-myristoyltransferase 1; NMT; NMT 1; Peptide N-myristoyltransferase 1; type I N-myristoyltransferase
Description (eCommerce)
NMT1 adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30419].
Description (Vector)
Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 (PubMed 1570339)).(supplied by OMIM, Nov 2008)
Gene ID
4836
Gene Name (eCommerce)
N-myristoyltransferase 1
Gene Name (Vector)
N-myristoyltransferase 1
Gene Symbol
NMT1
HGNC ID
HGNC:7857
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1491
ORF Size (bp)
1491 bp
Protein Name (eCommerce)
Glycylpeptide N-tetradecanoyltransferase 1
RefSeq ID
NM_021079
RefSeq Synonyms
NM_021079, BC006569, BC006538,
Research Areas
Acyltransferase,Apoptosis,Cytoskeleton,Host-Virus Interactions,Neurobiology,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,infectious_disease,neurobiology
Species
human
Target Sentence
NMT1 adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P30419].
UniGene ID
Hs.532790
UniProt ID (eCommerce)
P30419

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.