human NIT1 shRNA silencing AAV

Name 
AAV-h-NIT1-shRNA
Cat No 
shAAV-216735
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NIT1.

shAAV-216735
AAV-h-NIT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NIT1 (nitrilase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Description (Vector)
NIT1 encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
4817
Gene Name (Vector)
nitrilase 1
Gene Symbol
NIT1
HGNC ID
HGNC:7828
ORF Size (aa)
732
RefSeq ID
NM_005600
RefSeq Synonyms
NM_005600, NM_001185094, NM_001185093, NM_001185092, BC046149,
Species
human
UniGene ID
Hs.517342

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.