human NFYA shRNA silencing AAV

Name 
AAV-h-NFYA-shRNA
Cat No 
shAAV-216664
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NFYA.

shAAV-216664
AAV-h-NFYA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NFYA (nuclear transcription factor Y subunit alpha). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit
Description (eCommerce)
Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.
Description (Vector)
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.
Gene ID
4800
Gene Name (eCommerce)
nuclear transcription factor Y subunit alpha
Gene Name (Vector)
nuclear transcription factor Y subunit alpha
Gene Symbol
NFYA
HGNC ID
HGNC:7804
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
957
ORF Size (bp)
957 bp
Protein Name (eCommerce)
Nuclear transcription factor Y subunit alpha
RefSeq ID
NM_002505
RefSeq Synonyms
NM_021705, NM_002505, BC039244,
Research Areas
Antigen Processing and Presentation,Immunology,Metabolism/Metabolic Process,Protein Folding,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Wnt Signaling
Research Areas (Faceted)
cell_biology,immunology,metabolism,signal_transduction,transcription_translation
Species
human
Target Sentence
Nuclear transcription factor Y alpha (NF-YA) is a subunit of the NF-Y heterotrimeric complex composed of NF-YA, NF-YB, and NF-YC. The NF-Y factor binds the CCAAT box with high specificity and affinity, and it is the major CCAAT-binding activator. All 3 subunits are essential for CCAAT binding. NF-Y plays a role in regulating several cell-cycle regulated genes such as CDC2, CDC25A/B/C, cyclin A2, cyclin B1/B2, and E2F1.
UniGene ID
Hs.10441
UniProt ID (eCommerce)
P23511

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.