human NDUFA5 shRNA silencing AAV

Name 
AAV-h-NDUFA5-shRNA
Cat No 
shAAV-216482
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NDUFA5.

shAAV-216482
AAV-h-NDUFA5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NDUFA5 (NADH:ubiquinone oxidoreductase subunit A5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
B13; CI-13kB; CI-13KD-B; complex I 13kDa subunit B; complex I subunit B13; Complex I-13kD-B; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa; NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 5; NADH-ubiquinone oxidoreductase 13 kDa-B subunit; NUFM; type I dehydrogenase; ubiquinone reductase; UQOR13
Description (eCommerce)
NDUFA5 is a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. NDUFA5 localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone [taken from NCBI Entrez Gene (Gene ID: 4698)].
Description (Vector)
The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble 'iron-sulfur protein' (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.
Gene ID
4698
Gene Name (eCommerce)
NADH:ubiquinone oxidoreductase subunit A5
Gene Name (Vector)
NADH:ubiquinone oxidoreductase subunit A5
Gene Symbol
NDUFA5
HGNC ID
HGNC:7688
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
351
Protein Name (eCommerce)
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
RefSeq ID
NM_005000
RefSeq Synonyms
NM_005000, NM_001291304, NM_001282422, NM_001282421, NM_001282420, NM_001282419, BC070237, BC070236, BC020821, BC000813,
Research Areas
Alzheimer's Disease,Huntington's Disease,Metabolism/Metabolic Process,Mitochondrion,Neurobiology,Neurodegeneration,Parkinson's Disease
Research Areas (Faceted)
neurobiology,metabolism,cell_biology
Species
human
Target Sentence
NDUFA5 is a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. NDUFA5 localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone [taken from NCBI Entrez Gene (Gene ID: 4698)].
UniGene ID
Hs.651219
UniProt ID (eCommerce)
Q16718

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.