human MRPL18 shRNA silencing AAV

Name
AAV-h-MRPL18-shRNA

Cat No
shAAV-215788

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MRPL18.

shAAV-215788

AAV-h-MRPL18-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MRPL18 (mitochondrial ribosomal protein L18). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HSPC071; L18mt; MRP-L18

Description (Vector)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus.

Gene ID

29074

Gene Name (Vector)

mitochondrial ribosomal protein L18

Gene Symbol

MRPL18

HGNC ID

HGNC:14477

ORF Size (aa)

543

RefSeq ID

NM_014161

RefSeq Synonyms

NM_014161, NM_001318817, BC001623,

Species

human

UniGene ID

Hs.416998

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.