human MRPL14 shRNA silencing AAV

Name 
AAV-h-MRPL14-shRNA
Cat No 
shAAV-215783
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human MRPL14.

shAAV-215783
AAV-h-MRPL14-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MRPL14 (mitochondrial ribosomal protein L14). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
L14mt; L32mt; MRP-L14; MRP-L32; MRPL32; RMPL32; RPML32
Description (Vector)
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3.
Gene ID
64928
Gene Name (Vector)
mitochondrial ribosomal protein L14
Gene Symbol
MRPL14
HGNC ID
HGNC:14279
ORF Size (aa)
438
RefSeq ID
NM_032111
RefSeq Synonyms
NM_032111, NM_001318771, NM_001318770, NM_001318769, NM_001318768, NM_001318767, BC065005,
Species
human
UniGene ID
Hs.311190

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.