human MKL1 shRNA silencing AAV

Name
AAV-h-MKL1-shRNA

Cat No
shAAV-215497

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MKL1.

shAAV-215497

AAV-h-MKL1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MKL1 (megakaryoblastic leukemia (translocation) 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

basic, SAP and coiled-coil domain; BSAC; MAL; megakaryoblastic leukemia (translocation) 1; megakaryoblastic leukemia 1 protein; megakaryocytic acute leukemia protein; MKL; MKL/myocardin-like protein 1; MKL1; MRTF-A

Description (eCommerce)

MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.

Description (Vector)

MKL1 interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia.

Gene ID

57591

Gene Name (eCommerce)

myocardin related transcription factor A

Gene Name (Vector)

megakaryoblastic leukemia (translocation) 1

Gene Symbol

MKL1

HGNC ID

HGNC:14334

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2793

ORF Size (bp)

2793 bp

Protein Name (eCommerce)

MKL/myocardin-like protein 1

RefSeq ID

NM_020831

RefSeq Synonyms

NM_020831, NM_001318139, NM_001282662, NM_001282661, NM_001282660, BC141655, BC141653, BC115039,

Research Areas

Cancer,Cell Biology,Cytoskeleton,Rho Signaling,Signal Transduction,Transcription Factor/Regulator

Research Areas (Faceted)

cancer,cell_biology,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.654688

UniProt ID (eCommerce)

Q969V6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.