human MED22 shRNA silencing AAV

Name
AAV-h-MED22-shRNA

Cat No
shAAV-215254

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human MED22.

shAAV-215254

AAV-h-MED22-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MED22 (mediator complex subunit 22). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

MED24; surf-5; SURF5

Description (Vector)

MED22 is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.

Gene ID

6837

Gene Name (Vector)

mediator complex subunit 22

Gene Symbol

MED22

HGNC ID

HGNC:11477

ORF Size (aa)

423

RefSeq ID

NM_133640

RefSeq Synonyms

NM_181491, NM_133640, BC040111, BC024225,

Species

human

UniGene ID

Hs.78354

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.