human MARCH1 shRNA silencing AAV

Name
AAV-h-MARCH1-shRNA

Cat No
shAAV-215013

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human MARCH1.

shAAV-215013

AAV-h-MARCH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human MARCH1 (membrane associated ring-CH-type finger 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

MARCH-I; RNF171

Description (Vector)

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 (PubMed 14722266); Thibodeau et al., 2008 (PubMed 18389477); De Gassart et al., 2008 (PubMed 18305173)).(supplied by OMIM, Mar 2010)

Gene ID

55016

Gene Name (Vector)

membrane associated ring-CH-type finger 1

Gene Symbol

1-Mar

HGNC ID

HGNC:26077

ORF Size (aa)

819

ORF Size (bp)

819 bp

RefSeq ID

NM_017923

RefSeq Synonyms

NM_017923, NM_001166373, BC153124, BC148531,

Species

human

UniGene ID

Hs.696248

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.