human LIAS shRNA silencing AAV

Name 
AAV-h-LIAS-shRNA
Cat No 
shAAV-213914
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human LIAS.

shAAV-213914
AAV-h-LIAS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human LIAS (lipoic acid synthetase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD
Description (Vector)
LIAS belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
Gene ID
11019
Gene Name (Vector)
lipoic acid synthetase
Gene Symbol
LIAS
HGNC ID
HGNC:16429
ORF Size (aa)
1119
ORF Size (bp)
1119 bp
RefSeq ID
NM_006859
RefSeq Synonyms
NM_194451, NM_006859, NM_001278592, NM_001278591, NM_001278590, BC062751, BC023635,
Species
human
UniGene ID
Hs.550502

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.