human LGMN shRNA silencing AAV

Name
AAV-h-LGMN-shRNA

Cat No
shAAV-213874

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human LGMN.

shAAV-213874

AAV-h-LGMN-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human LGMN (legumain). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AEP; LGMN1; PRSC1

Description (Vector)

LGMN encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. LGMN has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Gene ID

5641

Gene Name (Vector)

legumain

Gene Symbol

LGMN

HGNC ID

HGNC:9472

ORF Size (aa)

1302

ORF Size (bp)

1302 bp

RefSeq ID

NM_005606

RefSeq Synonyms

NM_005606, NM_001008530, BC003061,

Species

human

UniGene ID

Hs.18069

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.