human HSD11B1 shRNA silencing AAV

Name
AAV-h-HSD11B1-shRNA

Cat No
shAAV-211557

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human HSD11B1.

shAAV-211557

AAV-h-HSD11B1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HSD11B1 (hydroxysteroid 11-beta dehydrogenase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

11-beta-HSD1; 11-DH; CORTRD2; HDL; HSD11; HSD11B; HSD11L; SDR26C1

Description (Vector)

HSD11B1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.

Gene ID

3290

Gene Name (Vector)

hydroxysteroid 11-beta dehydrogenase 1

Gene Symbol

HSD11B1

HGNC ID

HGNC:5208

ORF Size (aa)

879

ORF Size (bp)

879 bp

RefSeq ID

NM_181755

RefSeq Synonyms

NM_181755, NM_005525, NM_001206741, BC012593,

Species

human

UniGene ID

Hs.195040

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.