human HMBS shRNA silencing AAV

Name
AAV-h-HMBS-shRNA

Cat No
shAAV-211251

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human HMBS.

shAAV-211251

AAV-h-HMBS-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HMBS (hydroxymethylbilane synthase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Chester type; HMBS; Hydroxymethylbilane synthase; PBGD; PBG-D; PORC; porphobilinogen deaminase; porphyria, acute; pre-uroporphyrinogen synthase; UPS; uroporphyrinogen I synthase; uroporphyrinogen I synthetase

Description (eCommerce)

Hydroxymethylbilane synthase (HMBS) is a member of the hydroxymethylbilane synthase superfamily. HMBS is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane [taken from NCBI Entrez Gene (Gene ID: 3145)].

Description (Vector)

HMBS encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.

Gene ID

3145

Gene Name (eCommerce)

hydroxymethylbilane synthase

Gene Name (Vector)

hydroxymethylbilane synthase

Gene Symbol

HMBS

HGNC ID

HGNC:4982

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1086

ORF Size (bp)

1086 bp

Protein Name (eCommerce)

Porphobilinogen deaminase

RefSeq ID

NM_000190

RefSeq Synonyms

NM_001258209, NM_001258208, NM_001024382, NM_000190, BC019323, BC008149, BC000520,

Research Areas

Hypoxia,Metabolism/Metabolic Process,Neurobiology

Research Areas (Faceted)

cell_biology,metabolism,neurobiology

Species

human

Target Sentence

Hydroxymethylbilane synthase (HMBS) is a member of the hydroxymethylbilane synthase superfamily. HMBS is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane [taken from NCBI Entrez Gene (Gene ID: 3145)].

UniGene ID

Hs.82609

UniProt ID (eCommerce)

P08397

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.