human HIRA shRNA silencing AAV

Name
AAV-h-HIRA-shRNA

Cat No
shAAV-211046

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human HIRA.

shAAV-211046

AAV-h-HIRA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human HIRA (histone cell cycle regulator). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

DGCR1; TUP1; TUPLE1

Description (Vector)

HIRA encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. HIRA plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.

Gene ID

7290

Gene Name (Vector)

histone cell cycle regulator

Gene Symbol

HIRA

HGNC ID

HGNC:4916

ORF Size (aa)

3054

ORF Size (bp)

3054 bp

RefSeq ID

NM_003325

RefSeq Synonyms

NM_003325, BC039835,

Species

human

UniGene ID

Hs.474206

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.