human GNE shRNA silencing AAV

Name
AAV-h-GNE-shRNA

Cat No
shAAV-210109

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human GNE.

shAAV-210109

AAV-h-GNE-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GNE (glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

DMRV; GLCNE; IBM2; NM; Uae1

Description (Vector)

GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms.

Gene ID

10020

Gene Name (Vector)

glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase

Gene Symbol

GNE

HGNC ID

HGNC:23657

ORF Size (aa)

2169

ORF Size (bp)

2169 bp

RefSeq ID

NM_005476

RefSeq Synonyms

NM_005476, NM_001190388, NM_001190384, NM_001190383, NM_001128227, BC121179,

Species

human

UniGene ID

Hs.5920

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.