human GLDC shRNA silencing AAV

Name
AAV-h-GLDC-shRNA

Cat No
shAAV-209960

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human GLDC.

shAAV-209960

AAV-h-GLDC-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GLDC (glycine decarboxylase). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

GCE; GCSP; HYGN1

Description (Vector)

Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).

Gene ID

2731

Gene Name (Vector)

glycine decarboxylase

Gene Symbol

GLDC

HGNC ID

HGNC:4313

ORF Size (aa)

3063

ORF Size (bp)

3063 bp

RefSeq ID

NM_000170

RefSeq Synonyms

NM_000170, BC111995, BC111993,

Species

human

UniGene ID

Hs.584238

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.