human GEFT shRNA silencing AAV

Name 
AAV-h-GEFT-shRNA
Cat No 
shAAV-209803
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ARHGEF25.

shAAV-209803
AAV-h-GEFT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ARHGEF25 (Rho guanine nucleotide exchange factor 25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
GEFT; p63RhoGEF
Description (Vector)
Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
115557
Gene Name (Vector)
Rho guanine nucleotide exchange factor 25
Gene Symbol
ARHGEF25
HGNC ID
HGNC:30275
ORF Size (aa)
1425
ORF Size (bp)
1425 bp
RefSeq ID
NM_001111270
RefSeq Synonyms
NM_182947, NM_001111270, BC047559, BC012860,
Species
human
UniGene ID
Hs.61581

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.