human GCK shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human GCK.
shAAV-209742
AAV-h-GCK-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human GCK (glucokinase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
Description (Vector)
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Gene ID
2645
Gene Name (Vector)
glucokinase
Gene Symbol
GCK
HGNC ID
HGNC:4195
ORF Size (aa)
1398
ORF Size (bp)
1398 bp
RefSeq ID
NM_033507
RefSeq Synonyms
NM_033508, NM_033507, NM_000162, BC001890,
Species
human
UniGene ID
Hs.1270
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.