human FLII shRNA silencing AAV

Name 
AAV-h-FLII-shRNA
Cat No 
shAAV-209085
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human FLII.

shAAV-209085
AAV-h-FLII-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human FLII (FLII, actin remodeling protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
FLI; Fli1; flightless I actin binding protein; flightless I homolog; FLIL; protein flightless-1 homolog
Description (eCommerce)
Flightless-1 is the human homolog of the drosophila flightless (fli1), a gene required for proper development of flight muscles and essential to embryonic development. It is a member of the gelsolin family of actin-binding proteins, and is highly conserved among vertebrates and invertebrates. The actin-remodeling activity of flightless-1 may have multiple roles in the cell. Studies in transgenic mice indicate a role in wound healing that may involve the regulation of cell migration and proliferation. Flightless-1 has also been found to be associated with complexes involved in transcriptional regulation. It associates with CARM1, a protein arginine methyltransferase, and may function as a nuclear receptor coactivator via this interaction. It has also been shown to associate with the BAF53a subunit of the SWI/SNF-like chromatin remodeling complexes.
Description (Vector)
FLII encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Gene ID
2314
Gene Name (eCommerce)
FLII, actin remodeling protein
Gene Name (Vector)
FLII, actin remodeling protein
Gene Symbol
FLII
HGNC ID
HGNC:3750
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3810
ORF Size (bp)
3810 bp
Protein Name (eCommerce)
Protein flightless-1 homolog
RefSeq ID
NM_002018
RefSeq Synonyms
NM_002018, NM_001256265, NM_001256264, BC025300,
Research Areas
Cytoskeleton,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,transcription_translation
Species
human
Target Sentence
Flightless-1 is the human homolog of the drosophila flightless (fli1), a gene required for proper development of flight muscles and essential to embryonic development. It is a member of the gelsolin family of actin-binding proteins, and is highly conserved among vertebrates and invertebrates. The actin-remodeling activity of flightless-1 may have multiple roles in the cell. Studies in transgenic mice indicate a role in wound healing that may involve the regulation of cell migration and proliferation. Flightless-1 has also been found to be associated with complexes involved in transcriptional regulation. It associates with CARM1, a protein arginine methyltransferase, and may function as a nuclear receptor coactivator via this interaction. It has also been shown to associate with the BAF53a subunit of the SWI/SNF-like chromatin remodeling complexes.
UniGene ID
Hs.513984
UniProt ID (eCommerce)
Q13045

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.