human EP300 shRNA silencing AAV

Name 
AAV-h-EP300-shRNA
Cat No 
shAAV-207954
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human EP300.

shAAV-207954
AAV-h-EP300-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human EP300 (E1A binding protein p300). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
E1A-associated protein p300; E1A-binding protein, 300kD; histone acetyltransferase p300; histone butyryltransferase p300; histone crotonyltransferase p300; KAT3B; p300; p300 HAT; protein propionyltransferase p300; RSTS2
Description (eCommerce)
By sequence similarity p300 is related to CBP (CREB-binding protein) and functions as a histone acetyltransferase that can regulate transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha).
Description (Vector)
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
Gene ID
2033
Gene Name (eCommerce)
E1A binding protein p300
Gene Name (Vector)
E1A binding protein p300
Gene Symbol
EP300
HGNC ID
HGNC:3373
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
7242
ORF Size (bp)
7242 bp
Protein Name (eCommerce)
Histone acetyltransferase p300
RefSeq ID
NM_001429
RefSeq Synonyms
NM_001429, BC172280,
Research Areas
Acyltransferase,Apoptosis,Breast Cancer,Cancer,Cardiac Development,Cardiology,Cardiovascular,Cell Cycle,Estrogen Signaling,FoxO Signaling,Glioma,Hedgehog Signaling,HIF-1 Signaling,Histone Modification,Host-Virus Interactions,Huntington's Disease,Hypoxia,IFN Signaling,Immunology,Innate Immunity,Interleukin Signaling,JAK-STAT Signaling,Kidney Cancer,Metabolism/Metabolic Process,MicroRNAs in Cancer,Neurobiology,Neurodegeneration,Neurodevelopment,NFAT Signaling,Notch Signaling,Nuclear Receptor Coactivators,p53 Signaling,Prostate Cancer,SCF-KIT Signaling,Signal Transduction,TGF-beta Signaling,TNF Signaling,Transcription Factor/Regulator,Viral Carcinogenesis,Wnt Signaling,Zinc-finger
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation,immunology,infectious_disease,metabolism,neurobiology,signal_transduction,stem_cell_biology
Species
human
Target Sentence
By sequence similarity p300 is related to CBP (CREB-binding protein) and functions as a histone acetyltransferase that can regulate transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha).
UniGene ID
Hs.655211
UniProt ID (eCommerce)
Q09472

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.