human ENO1 shRNA silencing AAV

Name
AAV-h-ENO1-shRNA

Cat No
shAAV-207915

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ENO1.

shAAV-207915

AAV-h-ENO1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ENO1 (enolase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ENO1L1; HEL-S-17; MPB1; NNE; PPH

Description (Vector)

ENO1 encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.

Gene ID

2023

Gene Name (Vector)

enolase 1

Gene Symbol

ENO1

HGNC ID

HGNC:3350

ORF Size (aa)

1305

ORF Size (bp)

1305 bp

RefSeq ID

NM_001428

RefSeq Synonyms

NM_001428, NM_001201483, BC050642, BC027725, BC022545, BC015641, BC011130, BC009912, BC004458, BC001810,

Species

human

UniGene ID

Hs.517145

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.