human ECH1 shRNA silencing AAV

Name
AAV-h-ECH1-shRNA

Cat No
shAAV-207530

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ECH1.

shAAV-207530

AAV-h-ECH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ECH1 (enoyl-CoA hydratase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HPXEL

Description (Vector)

ECH1 encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Gene ID

1891

Gene Name (Vector)

enoyl-CoA hydratase 1

Gene Symbol

ECH1

HGNC ID

HGNC:3149

ORF Size (aa)

987

ORF Size (bp)

987 bp

RefSeq ID

NM_001398

RefSeq Synonyms

NM_001398, BC017408, BC011792,

Species

human

UniGene ID

Hs.196176

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.