human DOCK3 shRNA silencing AAV

Name
AAV-h-DOCK3-shRNA

Cat No
shAAV-207228

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human DOCK3.

shAAV-207228

AAV-h-DOCK3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DOCK3 (dedicator of cytokinesis 3). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

MOCA; PBP

Description (Vector)

DOCK3 is specifically expressed in the central nervous system (CNS). It encodes a member of the DOCK (dedicator of cytokinesis) family of guanine nucleotide exchange factors (GEFs). This protein, dedicator of cytokinesis 3 (DOCK3), is also known as modifier of cell adhesion (MOCA) and presenilin-binding protein (PBP). The DOCK3 and DOCK1, -2 and -4 share several conserved amino acids in their DHR-2 (DOCK homology region 2) domains that are required for GEF activity, and bind directly to WAVE proteins (Wiskott-Aldrich syndrome protein (WASP) family Verprolin-homologous proteins) via their DHR-1 domains. The DOCK3 induces axonal outgrowth in CNS by stimulating membrane recruitment of the WAVE complex and activating the small G protein Rac1. DOCK3 is associated with an attention deficit hyperactivity disorder-like phenotype by a complex chromosomal rearrangement.

Gene ID

1795

Gene Name (Vector)

dedicator of cytokinesis 3

Gene Symbol

DOCK3

HGNC ID

HGNC:2989

ORF Size (aa)

6090

ORF Size (bp)

6090 bp

RefSeq ID

NM_004947

RefSeq Synonyms

NM_004947, BC157127, BC156339,

Species

human

UniGene ID

Hs.476284

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.