human DNASE1 shRNA silencing AAV

Name
AAV-h-DNASE1-shRNA

Cat No
shAAV-207186

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human DNASE1.

shAAV-207186

AAV-h-DNASE1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DNASE1 (deoxyribonuclease 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

DNL1; DRNI

Description (Vector)

DNASE1 encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Gene ID

1773

Gene Name (Vector)

deoxyribonuclease 1

Gene Symbol

DNASE1

HGNC ID

HGNC:2956

ORF Size (aa)

849

ORF Size (bp)

849 bp

RefSeq ID

NM_005223

RefSeq Synonyms

NM_005223, BC029437,

Species

human

UniGene ID

Hs.629638

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.