human DIAPH1 shRNA silencing AAV

Name 
AAV-h-DIAPH1-shRNA
Cat No 
shAAV-206984
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DIAPH1.

shAAV-206984
AAV-h-DIAPH1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DIAPH1 (diaphanous related formin 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DFNA1; DIA1; Diaphanous-related formin-1; DRF1; hDIA1; LFHL1; protein diaphanous homolog 1; SCBMS
Description (eCommerce)
Diaphanous 1 is a member of the formin family of proteins that is responsible for actin polymerization and nucleation activity and the formation of linear, unbranched actin cables and stress fibers. Diaphanous 1 is a target of the small GTPase, Rho, and couples Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Diaphanous 1 has been linked to heritable nonsyndromic progressive hearing loss.
Description (Vector)
DIAPH1 is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Gene ID
1729
Gene Name (eCommerce)
diaphanous related formin 1
Gene Name (Vector)
diaphanous related formin 1
Gene Symbol
DIAPH1
HGNC ID
HGNC:2876
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3789
ORF Size (bp)
3789 bp
Protein Name (eCommerce)
Protein diaphanous homolog 1
RefSeq ID
NM_005219
RefSeq Synonyms
NM_005219, NM_001314007, NM_001079812, BC143413, BC117257,
Research Areas
Cell Cycle,Cytoskeleton,Immunology,Neurobiology,Rho Signaling,Signal Transduction,T-cell Receptor Signaling
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,immunology,neurobiology,signal_transduction
Species
human
Target Sentence
Diaphanous 1 is a member of the formin family of proteins that is responsible for actin polymerization and nucleation activity and the formation of linear, unbranched actin cables and stress fibers. Diaphanous 1 is a target of the small GTPase, Rho, and couples Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Diaphanous 1 has been linked to heritable nonsyndromic progressive hearing loss.
UniGene ID
Hs.529451
UniProt ID (eCommerce)
O60610

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.