human DENND1B shRNA silencing AAV

Name
AAV-h-DENND1B-shRNA

Cat No
shAAV-206866

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human DENND1B.

shAAV-206866

AAV-h-DENND1B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DENND1B (DENN domain containing 1B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

C1ORF18; C1orf218; FAM31B

Description (Vector)

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 (PubMed 20154091)).(supplied by OMIM, Nov 2010)

Gene ID

163486

Gene Name (Vector)

DENN domain containing 1B

Gene Symbol

DENND1B

HGNC ID

HGNC:28404

ORF Size (aa)

1191

ORF Size (bp)

1191 bp

RefSeq ID

NM_144977

RefSeq Synonyms

NM_144977, NM_001300858, NM_001195216, NM_001195215, BC063877, BC022561,

Species

human

UniGene ID

Hs.657779

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.