human DDX25 shRNA silencing AAV

Name
AAV-h-DDX25-shRNA

Cat No
shAAV-206746

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human DDX25.

shAAV-206746

AAV-h-DDX25-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DDX25 (DEAD-box helicase 25). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

GRTH

Description (Vector)

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The encoded protein is a gonadotropin-regulated and developmentally expressed testicular RNA helicase. It may serve to maintain testicular functions related to steroidogenesis and spermatogenesis.

Gene ID

29118

Gene Name (Vector)

DEAD-box helicase 25

Gene Symbol

DDX25

HGNC ID

HGNC:18698

ORF Size (aa)

1110

ORF Size (bp)

1110 bp

RefSeq ID

NM_013264

RefSeq Synonyms

NM_013264, NM_001330438, BC050360, BC035388,

Species

human

UniGene ID

Hs.420263

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.