human DCX shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human DCX.
shAAV-206688
AAV-h-DCX-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human DCX (doublecortin). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
DBCN; DC; doublecortex; doublin; lissencephalin-X; LISX; lis-X; neuronal migration protein doublecortin; SCLH; XLIS
Description (eCommerce)
Doublecortin is a microtubule-associated protein required for the initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Doublecortin directs neuronal migration by regulating the organization and stability of microtubules. In addition, doublecortin interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43602].
Description (Vector)
DCX encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ('double cortex' syndrome) in females and lissencephaly ('smooth brain' syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
1641
Gene Name (eCommerce)
doublecortin
Gene Name (Vector)
doublecortin
Gene Symbol
DCX
HGNC ID
HGNC:2714
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1083
ORF Size (bp)
1083 bp
Protein Name (eCommerce)
Neuronal migration protein doublecortin
RefSeq ID
NM_000555
RefSeq Synonyms
NM_178153, NM_178152, NM_178151, NM_001195553, NM_000555, BC027925,
Research Areas
Axon Guidance,Brain Development,Cytoskeleton,Epilepsy,Neurobiology,Neurodevelopment,Neurogenesis
Research Areas (Faceted)
neurobiology,developmental_biology,cell_biology
Species
human
Target Sentence
Doublecortin is a microtubule-associated protein required for the initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. Doublecortin directs neuronal migration by regulating the organization and stability of microtubules. In addition, doublecortin interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43602].
UniGene ID
Hs.34780
UniProt ID (eCommerce)
O43602
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.